کشت بافت میخک


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 این مطلب در ادامه مطلب کشت بافت میخک ارسال می شود تا اطلاعات کشت بافت میخک کامل شود. در ادامه مطلب کشت بافت میخک مشکلاتی که در کشت بافت این گیاه به انها برخورد خواهید کرد را با ذکر روش کاهش این مشکلات ذکر می کنم . برای پیدا کردن پروتوکول های مناسب برای تکثیر […]


محیط کشت WPM


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محیط کشت Lloyd & McCown’s Woody Plant Medium این محیط کشت در ابتدا برای کشت نوک ساقه گیاه Kalmia latifolia از روی محیط کشت MS  توسط  Lloyd و  McCown ساخته شد و بعد از ان با موفقیت برای بسیاری از گیاهان چوبی مورد استفاده قرار گرفت . نیترات پتاسیم در این محیط کشت حذف شده […]


محیط کشت Nitsch


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محیط کشت Nitsch در سال ۱۹۶۹ توسط Nitsch  برای کشت میکرواسپور تنباکو و تولید گیاه هاپلوئید ساخته شد بعداً این محیط کشت یکی از محیط کشت های پرکاربرد در کشت بساک و میکرواسپور در گیاهان مختلف شد . فرمول محیط کشت


محیط کشت N6


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محیط کشت Chu’s N6 یک محیط کشت تقریبا تخصصی برای کشت بساک و گرده در غلات و بخصوص برنج در سال ۱۹۷۵ توسط  Chu ساخته شده است . این محیط کشت ابتدا برای القا ، رشد و تمایز کالوس در کشت گرده برنج ساخته و استفاده شد . در این محیط کشت سولفات امونیوم جایگزین […]


محیط کشت LS


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محیط کشت LS  یا linsmaier and skoog Medium این محیط کشت توسط فولکه کارل اسکوگ و  Elfriede M. Linsmaier در سال ۱۹۶۵ ساخته شد. ابتدا برای کشت تنباکو استفاده شد و بعدا برای گیاهان دیگر.این محیط کشت دارای ماکرو و میکروالمنتهای محیط کشت ام اس معمولی را دارد ولی ویتامینها توسط این دو محقق تغییر […]


کشت بافت در خانه : مواد مورد نیاز


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کشت بافت در خانه : مواد مورد نیاز بعد از مدتها ازمایش روی روش های کشت بافت در خانه اکنون روشی ساده و ارزان را برای کاربران سایت اماده کردم تا از این به بعد هر کسی بخواهد بتواند با موفقیت کشت بافت را در خانه اموزش ببیند .اینگونه است که که می توان این […]


محیط کشت Gamborg B5


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محیط کشت Gamborg B5 نیز یکی از پرکاربردترین محیط های کشت بافت گیاهی می باشد که در کشت بافت تجاری و تحقیقاتی مورد استفاده قرار می گیرد .از نظر ترکیبات با محیط کشت MS در مقادیر مواد مورد استفاده تفاوت دارد و فاقد Glycine است ولی دارای تیامین بیشتری از محیط کشت MS است .از […]


محیط کشت MS یا Murashige and Skoog


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محیط کشت MS شاید پرکاربردترین محیط کشت بافت گیاهی در حال حاضر باشد و از ان برای کشت بافت بسیاری از گونه های گیاهی با موفقیت استفاده شده است و مرجع تولید چند نوع محیط کشت دیگر نیز شده است مانند محیط کشت LS که با کاهش مقدار مواد مورد استفاده در محیط کشت MS […]