محیط کشت WPM


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محیط کشت Lloyd & McCown’s Woody Plant Medium

این محیط کشت در ابتدا برای کشت نوک ساقه گیاه Kalmia latifolia از روی محیط کشت MS  توسط  Lloyd و  McCown ساخته شد و بعد از ان با موفقیت برای بسیاری از گیاهان چوبی مورد استفاده قرار گرفت .

نیترات پتاسیم در این محیط کشت حذف شده و سولفات پتاسیم افزوده شده . آمونیوم به یک چهارم مقدار موجود در محیط کشت MS  کاهش پیدا کرده و نیتروژن نیز از طریق کلسیم به محیط کشت افزوده می شود

فرمول محیط کشت

Components mg/L
Ammonium Nitrate (NH4NO3) 400.0000
Boric Acid (H3BO3) 6.2000
Calcium Chloride, Anhydrous (CaCl2) 72.5000
Calcium Nitrate, Tetrahydrate (Ca(NO3)2.4H2O) 386.0000
Cupric Sulfate, Pentahydrate (CuSO4.5H2O) 0.2500
EDTA, Disodium, Dihydrate (C10H14N2Na2O8.2H2O) 37.3000
Ferrous Sulfate, Heptahydrate (FeSO4.7H2O) 27.8500
Magnesium Sulfate, Anhydrous (MgSO4) 180.7000
Manganese Sulfate, Monohydrate (MnSO4.H2O) 22.3000
Molybdic Acid Sodium Salt, Dihydrate (Na2MoO4.2H2O) 0.2500
Myo-Inositol (C6H12O6) 100.0000
Nicotinic Acid (C6H5NO2) 0.5000
Potassium Phosphate, Monobasic, Anhydrous (KH2PO4) 170.0000
Potassium Sulfate, Anhydrous (K2SO4) 990.0000
Pyridoxine, Hydrochloride (C8H11NO3.HCl) 0.5000
Zinc Sulfate, Heptahydrate (ZnSO4.7H2O) 8.6000

مقدار مورد استفاده از محیط کشت در هر لیتر اب مقطر : ۲٫۴۱ گرم

دریافت فایل PDF فرمول محیط کشت (در صفحه جدید باز می شود)

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۲ جواب برای “محیط کشت WPM”


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    ضمن سلام به حضور اساتید عزیز
    در یو تویوپ فیلم هایی دیده ام که از انتهای شاخه های درختان به نام لا به لا گذاری هوا وبریدن پوست قسمتی از شاخه وبا کو کو پیت آن را محکم بسته وبعد چندهفته ریشه می کند .
    ۱ آیا درختانی چون گیلاس وگردو ویا هر درخت دیگری را می توان اینچنین زیاد کرد
    ۲ در چنین صورتی چه زمان باید این را انجام داد . توضیح دهید ممنون سپاسگزار میشوم

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    سلام

    منظور شما Air Layering است متاسفانه معادل فارسی این تکنیک اصلا مفهوم ان را نمی رساند ولی در هر صورت معادل ان خوابانیدن هوایی می باشد .از این تکنیک برای تکثیر گیاهان چوبی که نیاز به پیوند ندارند و گیاهانی که از طریق قلمه بسختی تکثیر می شوند استفاده می شود .برخی از درختان به دلایلی باید به پایه های مناسب پیوند زده شوند تا بخوبی رشد کرده و محصول دهند و واضح است که از روش خوابانیدن هوایی نمی شود برای تکثیر این گونه درختان استفاده کرد ولی در صورتی که درخت مورد نظر نیاز به پیوند ندارد می شود از تکنیک خوابانیدن هوایی برای تکثیر این درختان استفاده کرد .
    زمان مناسب برای درختان همیشه سبز فصل بهار است ولی درختان برگریز می توانند در تمام فصول با این روش تکثیر شوند.البته فصل زمستان بخاطر سردی زیاد مناسب نیست .
    معمولا درخت گیلاس و گردو پیوند زده می شوند ولی برخی ارقام نیز ممکن است از طریق قلمه هم خوب محصول دهند و خوابانیدن هوایی برای ارقامی که بدون پیوند خوب محصول می دهند قابل استفاده است .
    موفق باشید
    محمد صادق باقری

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