محیط کشت Gamborg B5


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محیط کشت Gamborg B5 نیز یکی از پرکاربردترین محیط های کشت بافت گیاهی می باشد که در کشت بافت تجاری و تحقیقاتی مورد استفاده قرار می گیرد .از نظر ترکیبات با محیط کشت MS در مقادیر مواد مورد استفاده تفاوت دارد و فاقد Glycine است ولی دارای تیامین بیشتری از محیط کشت MS است .از این محیط کشت نیز می توان برای منظور های مختلف تجاری و تحقیقاتی استفاده کرد البته گونه های مختلف و حتی ارقام مختلف یک گونه ممکن است از نظر میزان نیاز به برخی عناصر تفاوتهایی باشد که باید در تحقیقات مورد بررسی قرار گیرد و محیط کشت برای رقم مورد نظر بهینه سازی شود ولی این تغییرات بیشتر برای افزایش بهره وری انجام می شود و ارقام مختلف در یک محیط کشت بدون تغییر می توانند کشت شوند فقط میزان تکثیر در حد قابل قبولی نخواهد بود .

در هر حال کار تحقیقات در مورد تغییرات در فرمول محیط کشت وقت گیر و هزینه بردار است که برای یک فرد آماتور مناسب نیست و امکانات یک فرد آماتور نیز برای این کار مناسب نیست پس بهتر است قبل از تهیه محیط کشت در مورد کشت بافت گیاه مورد نظر اطلاعاتی جمع اوری کند و با آگاهی از محیط کشت مناسب اقدام به خرید محیط کشت آماده کند .

فرمول محیط کشت Gamborg B5 به صورت زیر است

Components mg/L
Ammonium Sulfate ((NH4)2SO4) ۱۳۴٫۰۰۰۰
Boric Acid (H3BO3) ۳٫۰۰۰۰
Calcium Chloride, Anhydrous (CaCl2) ۱۱۳٫۲۴۰۰
Cobalt Chloride, Hexahydrate (CoCl2 · ۶H2O) ۰٫۰۲۵۰
Cupric Sulfate, Pentahydrate (CuSO4 · ۵H2O) ۰٫۰۲۵۰
EDTA, Disodium, Dihydrate (C10H14N2Na2O8 · ۲H2O) ۳۷٫۲۶۰۰
Ferrous Sulfate, Heptahydrate (FeSO4•۷H2O) ۲۷٫۸۰۰۰
Magnesium Sulfate, Anhydrous (MgSO4) ۱۲۲٫۰۹۰۰
Manganese Sulfate, Monohydrate (MnSO4 · H2O) ۱۰٫۰۰۰۰
Molybdic Acid Sodium Salt, Dihydrate (Na2MoO4 · ۲H2O) ۰٫۲۵۰۰
Myo-Inositol (C6H12O6) ۱۰۰٫۰۰۰۰
Nicotinic Acid (C6H5NO2) ۱٫۰۰۰۰
Potassium Iodide (KI) ۰٫۷۵۰۰
Potassium Nitrate (KNO3) ۲۵۰۰٫۰۰۰۰
Pyridoxine, Hydrochloride (C8H11NO3 · HCl) ۱٫۰۰۰۰
Sodium Phosphate, Monobasic, Monohydrate (NaH2PO4 · H2O) ۱۵۰٫۰۸۰۰
Thiamine, Hydrochloride (C12H17ClN4OS · HCl) ۱۰٫۰۰۰۰
Zinc Sulfate, Heptahydrate (ZnSO4•۷H2O) ۲٫۰۰۰۰

مقدار مورد استفاده از محیط کشت در هر لیتر اب مقطر : ۳٫۲۱ گرم

برای افراد آماتور می توان از این محیط کشت برای تکثیر لیلیوم , بگونیا و بنفشه آفریقایی استفاده کرد .

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محیط کشت Gamborg B5, 6.9 out of 10 based on 16 ratings

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    با سلام و تشکر
    لطفا در خصوص تهیه محیط کشت آماده مرا راهنمایی فرمایید .متشکرم

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